Hence, the medical personnel, laboratory people, and health care providers should have better knowledge regarding these techniques to prevent the spread of these pathogens. Currently, there is an increase in frequency of newly emerging and multidrug-resistant pathogens in all health care centers for which only few/if any treatments are available. Every hospital should have its own guidelines of sterilizing and disinfecting items on the basis of their intended use of medical devices and associated infections. The health care providers are equally responsible for the reduction and elimination of such infections. 3, 4, 5, 6, 7 Hence, adequate decontamination techniques for medical and surgical devices are needed in all the health care facilities. 1, 2 Many infections were reported throughout the world due to use of contaminated endoscopes. A number of outbreaks and infections were reported in the hospital setup because of improperly sterilized devices. Moreover, there is chance of transmission of infection from patient to patient from patient or to health care personnel, and vice versa or from the environment to the patient (e.g., Pseudomonas aeruginosa, Acinetobacter spp.) through the improper sterilized or disinfected devices. The medical device or the surgical instrument that comes in contact with the sterile tissue or the mucus membrane of the patient during the various processes is associated with increased risk of introduction of pathogens into the patient’s body. Even more number of invasive procedures are being performed in different health care facilities. Every day, a number of hospitals are performing various surgical procedures. Sterilization and disinfection are the basic components of hospital infection control activities.

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